Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha

Running title: Branched-chain alcohols production in engineered Ralstonia eutropha

BIOENERGYAND BIOFUELS Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha

Wild-type Ralstonia eutropha H16 produces polyhydroxybutyrate (PHB) as an intracellular carbon storage material during nutrient stress in the presence of excess carbon. In this study, the excess carbon was redirected in engineered strains from PHB storage to the production of isobutanol and 3-methyl-1-butanol (branched-chain higher alcohols). These branched-chain higher alcohols can directly su...

Influence of Matric Potential on Survival and Activity of Genetically Engineered Ralstonia eutropha H850Lr

Although the application of biodegradative genetically engineered micro organisms (GEMs) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. Lux-marked Ralstonia eutropha H850Lr (formerly Alcaligenes eutrophus H850Lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...

Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains.

The polyhydroxyalkanoate (PHA) copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has been shown to have potential to serve as a commercial bioplastic. Synthesis of P(HB-co-HHx) from plant oil has been demonstrated with recombinant Ralstonia eutropha strains expressing heterologous PHA synthases capable of incorporating HB and HHx into the polymer. With these strains, howeve...

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM PALMITIC ACID USING Ralstonia eutropha BY FED BATCH CULTURE

Polyhydroxyalkanoates (PHA’s) are carbon and energy storage compounds which accumulate intracellulary as granules in certain bacteria during unbalanced growth and in the presence of an excess carbon source. PHAs were well studied regarding their promising applications, physical, chemical and biological properties. PHAs are biodegradable, biocompatible, have good material properties, renewable a...